Resolution of Myocardial Myosin Light Chains by Two-Dimensional Gel Electrophoresis

Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis

Human HLA-DR antigens were immunoprecipitated from Nonidet P-40 extracts of [35S]methionine-labeled B lymphoblastoid cell lines and compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). Two-dimensional (2-D) gel analyses, combining SDS-PAGE in the first dimension and IEF in the second dimension, revealed that the heavy (alpha) and light...

Two-dimensional Gel Electrophoresis

Identification by Two-Dimensional Gel Electrophoresis

We have previously identified proteins in fractions of culture filtrate ofMycobacterium tuberculosis with the capacity to induce cytokine production in monocytes, by using a technique we defined as "monocyte Western blotting" (immunoblotting). In this series of experiments, we have extended this technique to two-dimensional gel electrophoresis and have identified a novel 58-kDa protein ofM. tub...

Immunogens of Brucella Abortus S19 Identified By Two-Dimensional Gel Electrophoresis and Immunoblotting

Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction b...

Resolution of microsomal membrane proteins by two-dimensional gel electrophoresis [proceedings].

the citrate medium described by Halestrap (1976) was used, since this generated a pH gradient (alkaline inside) which aided pyruvate and lactate accumulation. In other experiments buffered saline was used as described by Spencer & Lehninger (1976) in order to resemble the physiological situation more closely. Details of the conditions are given in the legends to the Figure and Table. In Fig. 1 ...